Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

Jin Tang Chen; Torng Sen Lin; Kuan Chih Chow; Hsuan Hua Huang; Shiow Her Chiou; Shu Fen Chiang; Hong Chen Chen; Tzu Lin Chuang; Tze Yi Lin; Chih Yi Chen

doi:10.1165/rcmb.2005-0117OC

Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

Jin Tang Chen, Torng Sen Lin, Kuan Chih Chow^*, Hsuan Hua Huang, Shiow Her Chiou, Shu Fen Chiang, Hong Chen Chen, Tzu Lin Chuang, Tze Yi Lin, Chih Yi Chen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HCF expression in ATII cells and lung cancer cells.

Original language	English
Pages (from-to)	264-273
Number of pages	10
Journal	American journal of respiratory cell and molecular biology
Volume	34
Issue number	3
DOIs	https://doi.org/10.1165/rcmb.2005-0117OC
State	Published - Mar 2006

Keywords

Immunohistochemistry
In situ hybridization
Microarray
Suppression subtractive hybridization
Type II pneumocyte

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10.1165/rcmb.2005-0117OC

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Chen, J. T., Lin, T. S., Chow, K. C., Huang, H. H., Chiou, S. H., Chiang, S. F., Chen, H. C., Chuang, T. L., Lin, T. Y., & Chen, C. Y. (2006). Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells. American journal of respiratory cell and molecular biology, 34(3), 264-273. https://doi.org/10.1165/rcmb.2005-0117OC

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title = "Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells",

abstract = "We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HCF expression in ATII cells and lung cancer cells.",

keywords = "Immunohistochemistry, In situ hybridization, Microarray, Suppression subtractive hybridization, Type II pneumocyte",

author = "Chen, {Jin Tang} and Lin, {Torng Sen} and Chow, {Kuan Chih} and Huang, {Hsuan Hua} and Chiou, {Shiow Her} and Chiang, {Shu Fen} and Chen, {Hong Chen} and Chuang, {Tzu Lin} and Lin, {Tze Yi} and Chen, {Chih Yi}",

year = "2006",

month = mar,

doi = "10.1165/rcmb.2005-0117OC",

language = "English",

volume = "34",

pages = "264--273",

journal = "American journal of respiratory cell and molecular biology",

issn = "1044-1549",

publisher = "American Thoracic Society",

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TY - JOUR

T1 - Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

AU - Chen, Jin Tang

AU - Lin, Torng Sen

AU - Chow, Kuan Chih

AU - Huang, Hsuan Hua

AU - Chiou, Shiow Her

AU - Chiang, Shu Fen

AU - Chen, Hong Chen

AU - Chuang, Tzu Lin

AU - Lin, Tze Yi

AU - Chen, Chih Yi

PY - 2006/3

Y1 - 2006/3

N2 - We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HCF expression in ATII cells and lung cancer cells.

AB - We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HCF expression in ATII cells and lung cancer cells.

KW - Immunohistochemistry

KW - In situ hybridization

KW - Microarray

KW - Suppression subtractive hybridization

KW - Type II pneumocyte

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U2 - 10.1165/rcmb.2005-0117OC

DO - 10.1165/rcmb.2005-0117OC

M3 - Article

C2 - 16254251

AN - SCOPUS:33644623549

SN - 1044-1549

VL - 34

SP - 264

EP - 273

JO - American journal of respiratory cell and molecular biology

JF - American journal of respiratory cell and molecular biology

IS - 3

ER -

Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells

Abstract

Keywords

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