Chiral Structured Illumination Microscopy

Shiang-Yu Huang; Jiwei Zhang; Christian Karras; Ronny Forster; Rainer Heintzmann; Jer-Shing Huang

doi:10.1021/acsphotonics.0c01360

Chiral Structured Illumination Microscopy

Shiang-Yu Huang, Jiwei Zhang, Christian Karras, Ronny Forster, Rainer Heintzmann, Jer-Shing Huang

Department of Electrophysics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the circular dichroism dependent fluorescence is detected. With image reconstruction, the spatial distribution of the chiral domains can be obtained at subdiffraction-limited resolution. We theoretically demonstrate this method and discuss the feasibility of using an optical chirality engineering approach based on far-field optics.

Original language	English
Pages (from-to)	130-134
Number of pages	5
Journal	ACS Photonics
Volume	8
Issue number	1
DOIs	https://doi.org/10.1021/acsphotonics.0c01360
State	Published - 20 Jan 2021

Keywords

chiral imaging
structured illumination microscopy
optical chirality
circular dichroism
super resolution
CIRCULAR-DICHROISM SPECTROSCOPY
PLANE-WAVES
POLYFLUORENE
GENERATION
EXCITATION
RESOLUTION

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10.1021/acsphotonics.0c01360

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title = "Chiral Structured Illumination Microscopy",

abstract = "We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the circular dichroism dependent fluorescence is detected. With image reconstruction, the spatial distribution of the chiral domains can be obtained at subdiffraction-limited resolution. We theoretically demonstrate this method and discuss the feasibility of using an optical chirality engineering approach based on far-field optics.",

keywords = "chiral imaging, structured illumination microscopy, optical chirality, circular dichroism, super resolution, CIRCULAR-DICHROISM SPECTROSCOPY, PLANE-WAVES, POLYFLUORENE, GENERATION, EXCITATION, RESOLUTION",

author = "Shiang-Yu Huang and Jiwei Zhang and Christian Karras and Ronny Forster and Rainer Heintzmann and Jer-Shing Huang",

year = "2021",

month = jan,

day = "20",

doi = "10.1021/acsphotonics.0c01360",

language = "English",

volume = "8",

pages = "130--134",

journal = "ACS Photonics",

issn = "2330-4022",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Chiral Structured Illumination Microscopy

AU - Huang, Shiang-Yu

AU - Zhang, Jiwei

AU - Karras, Christian

AU - Forster, Ronny

AU - Heintzmann, Rainer

AU - Huang, Jer-Shing

PY - 2021/1/20

Y1 - 2021/1/20

N2 - We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the circular dichroism dependent fluorescence is detected. With image reconstruction, the spatial distribution of the chiral domains can be obtained at subdiffraction-limited resolution. We theoretically demonstrate this method and discuss the feasibility of using an optical chirality engineering approach based on far-field optics.

AB - We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the circular dichroism dependent fluorescence is detected. With image reconstruction, the spatial distribution of the chiral domains can be obtained at subdiffraction-limited resolution. We theoretically demonstrate this method and discuss the feasibility of using an optical chirality engineering approach based on far-field optics.

KW - chiral imaging

KW - structured illumination microscopy

KW - optical chirality

KW - circular dichroism

KW - super resolution

KW - CIRCULAR-DICHROISM SPECTROSCOPY

KW - PLANE-WAVES

KW - POLYFLUORENE

KW - GENERATION

KW - EXCITATION

KW - RESOLUTION

U2 - 10.1021/acsphotonics.0c01360

DO - 10.1021/acsphotonics.0c01360

M3 - Article

SN - 2330-4022

VL - 8

SP - 130

EP - 134

JO - ACS Photonics

JF - ACS Photonics

IS - 1

ER -

Chiral Structured Illumination Microscopy

Abstract

Keywords

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