Characterization of auto-fluorescence urine crystals from gout patients using confocal microscopy and micro-Raman system for urolithiasis prediction

Zih Ting Chen, Chih Hao Wang, Huihua Kenny Chiang*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

The major etiologies of gout and kidney stone formation are similar hyperuremia, which is caused by unbalanced uric acid production/exertion. And gout is one of the important risk factor that promotes kidney stone formation. It's worth noting that crystals retension/aggregation is the early stage of stone formation. However, the mechanism between gout and crystalluria remains unclear. Our previous studies found good correlation between auto-fluorescent crystals and urolithiasis. Higher proportion of auto-fluorescent crystalluria in gout patients were also noted. It this paper, we demonstrated characterization of auto-fluorescent urinary crystals in gout patients by micro-Raman system and laser confocal microscopy. Our purpose is to figure out the mechanism among gout flare, urolithiasis and auto-fluorescent crystal retention. And the results supported that fluorophore inside the crystals may be a powerful indicator for crystals retention. Further studies are required to validate our hypothesis.

Original languageEnglish
Title of host publicationBiomedical Spectroscopy, Microscopy, and Imaging
EditorsJurgen Popp, Csilla Gergely
PublisherSPIE
ISBN (Electronic)9781510634909
DOIs
StatePublished - 2020
EventBiomedical Spectroscopy, Microscopy, and Imaging 2020 - Virtual, Online, France
Duration: 6 Apr 202010 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11359
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceBiomedical Spectroscopy, Microscopy, and Imaging 2020
Country/TerritoryFrance
CityVirtual, Online
Period6/04/2010/04/20

Keywords

  • Auto-fluorescence
  • Gout
  • Laser confocal scanning microscopy(CLSM)
  • Micro-Raman spectroscopy(MRS)
  • Urine crystals
  • Urolithiasis

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