Characterization of a fiber bundle-based real-time ultrasound/photoacoustic imaging system and its in vivo functional imaging applications

He Leng, Yuhling Wang, De Fu Jhang, Tsung Sheng Chu, Chia Hui Tsao, Chia Hua Tsai, Steven Giamundo, You Yin Chen, Kuang Wen Liao, Chiung Cheng Chuang, Tzong Rong Ger, Li Tzong Chen, Lun De Liao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Photoacoustic (PA) imaging is an attractive technology for imaging biological tissues because it can capture both functional and structural information with satisfactory spatial resolution. Current commercially available PA imaging systems are limited by their bulky size or inflexible user interface. We present a new handheld real-time ultrasound/photoacoustic imaging system (HARP) consisting of a detachable, high-numerical-aperture (NA) fiber bundle-based illumination system integrated with an array-based ultrasound (US) transducer and a data acquisition platform. In this system, dierent PA probes can be used for dierent imaging applications by switching the transducers and the corresponding jackets to combine the fiber pads and transducer into a single probe. The intuitive user interface is a completely programmable MATLAB-based platform. In vitro phantom experiments were conducted to test the imaging performance of the developed PA system. Furthermore, we demonstrated (1) in vivo brain vasculature imaging, (2) in vivo imaging of real-time stimulus-evoked cortical hemodynamic changes during forepaw electrical stimulation, and (3) in vivo imaging of real-time cerebral pharmacokinetics in rats using the developed PA system. The overall purpose of this design concept for a customizable US/PA imaging system is to help overcome the diverse challenges faced by medical researchers performing both preclinical and clinical PA studies.

Original languageEnglish
Article number820
Issue number12
StatePublished - Dec 2019


  • Fiber bundle-based illumination
  • Hemoglobin oxygenation saturation
  • In vivo imaging
  • Photoacoustic (PA)
  • Ultrasound (US)


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