Characteristics of subgingival calculus detection by multiphoton fluorescence microscopy

Oi Hong Tung, Shyh Yuan Lee, Yu Lin Lai, How Foo Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Subgingival calculus has been recognized as a major cause of periodontitis, which is one of the main chronic infectious diseases of oral cavities and a principal cause of tooth loss in humans. Bacteria deposited in subgingival calculus or plaque cause gingival inflammation, function deterioration, and then periodontitis. However, subgingival calculus within the periodontal pocket is a complicated and potentially delicate structure to be detected with current dental armamentaria, namely dental x-rays and dental probes. Consequently, complete removal of subgingival calculus remains a challenge to periodontal therapies. In this study, the detection of subgingival calculus employing amultiphoton autofluorescence imaging method was characterized in comparison with a one-photon confocal fluorescence imaging technique. Feasibility of such a system was studied based on fluorescence response of gingiva, healthy teeth, and calculus with and without gingiva covered. The multiphoton fluorescence technology perceived the tissue-covered subgingival calculus that cannot be observed by the onephoton confocal fluorescence method.

Original languageEnglish
Article number066017
JournalJournal of Biomedical Optics
Issue number6
StatePublished - Jun 2011


  • Enamel
  • Gingiva
  • Multiphoton fluorescence
  • One-photon fluorescence
  • Subgingival calculus
  • Subgingival plaque


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