Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu 1-32 from HIV-1

Winfried Römer; Yuen H. Lam; Dahlia Fischer; Anthony Watts; Wolfgang B. Fischer; Petra Göring; Ralf B. Wehrspohn; Ulrich Gösele; Claudia Steinem

doi:10.1021/ja0451970

Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu _1-32 from HIV-1

Winfried Römer, Yuen H. Lam, Dahlia Fischer, Anthony Watts, Wolfgang B. Fischer, Petra Göring, Ralf B. Wehrspohn, Ulrich Gösele, Claudia Steinem^*

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We report for the first time on pore-suspending lipid bilayers, which we call micro-black lipid membranes (micro-BLMs), based on a highly ordered macroporous silicon array. Micro-BLMs were established by first functionalizing the backside porous silicon surface with gold and then chemisorbing 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol followed by spreading 1,2-diphytanoyl-sn-glycero-3-phosphocholine dissolved in n-decane. Impedance spectroscopy revealed the formation of single lipid bilayers confirmed by a mean specific capacitance of 0.6 ± 0.2 μF/cm². Membrane resistances were in the GΩ-regime and beyond. The potential of the system for single channel recordings was demonstrated by inserting the transmembrane domain of the HIV-1 accessory peptide Vpu_1-32, which forms helix bundles with characteristic opening states. We elucidated different amilorides as potential drugs to inhibit channel activity of Vpu.

Original language	English
Pages (from-to)	16267-16274
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	126
Issue number	49
DOIs	https://doi.org/10.1021/ja0451970
State	Published - 15 Dec 2004

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title = "Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu 1-32 from HIV-1",

abstract = "We report for the first time on pore-suspending lipid bilayers, which we call micro-black lipid membranes (micro-BLMs), based on a highly ordered macroporous silicon array. Micro-BLMs were established by first functionalizing the backside porous silicon surface with gold and then chemisorbing 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol followed by spreading 1,2-diphytanoyl-sn-glycero-3-phosphocholine dissolved in n-decane. Impedance spectroscopy revealed the formation of single lipid bilayers confirmed by a mean specific capacitance of 0.6 ± 0.2 μF/cm2. Membrane resistances were in the GΩ-regime and beyond. The potential of the system for single channel recordings was demonstrated by inserting the transmembrane domain of the HIV-1 accessory peptide Vpu1-32, which forms helix bundles with characteristic opening states. We elucidated different amilorides as potential drugs to inhibit channel activity of Vpu.",

author = "Winfried R{\"o}mer and Lam, {Yuen H.} and Dahlia Fischer and Anthony Watts and Fischer, {Wolfgang B.} and Petra G{\"o}ring and Wehrspohn, {Ralf B.} and Ulrich G{\"o}sele and Claudia Steinem",

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doi = "10.1021/ja0451970",

language = "English",

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T2 - Vpu 1-32 from HIV-1

AU - Römer, Winfried

AU - Lam, Yuen H.

AU - Fischer, Dahlia

AU - Watts, Anthony

AU - Fischer, Wolfgang B.

AU - Göring, Petra

AU - Wehrspohn, Ralf B.

AU - Gösele, Ulrich

AU - Steinem, Claudia

PY - 2004/12/15

Y1 - 2004/12/15

N2 - We report for the first time on pore-suspending lipid bilayers, which we call micro-black lipid membranes (micro-BLMs), based on a highly ordered macroporous silicon array. Micro-BLMs were established by first functionalizing the backside porous silicon surface with gold and then chemisorbing 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol followed by spreading 1,2-diphytanoyl-sn-glycero-3-phosphocholine dissolved in n-decane. Impedance spectroscopy revealed the formation of single lipid bilayers confirmed by a mean specific capacitance of 0.6 ± 0.2 μF/cm2. Membrane resistances were in the GΩ-regime and beyond. The potential of the system for single channel recordings was demonstrated by inserting the transmembrane domain of the HIV-1 accessory peptide Vpu1-32, which forms helix bundles with characteristic opening states. We elucidated different amilorides as potential drugs to inhibit channel activity of Vpu.

AB - We report for the first time on pore-suspending lipid bilayers, which we call micro-black lipid membranes (micro-BLMs), based on a highly ordered macroporous silicon array. Micro-BLMs were established by first functionalizing the backside porous silicon surface with gold and then chemisorbing 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol followed by spreading 1,2-diphytanoyl-sn-glycero-3-phosphocholine dissolved in n-decane. Impedance spectroscopy revealed the formation of single lipid bilayers confirmed by a mean specific capacitance of 0.6 ± 0.2 μF/cm2. Membrane resistances were in the GΩ-regime and beyond. The potential of the system for single channel recordings was demonstrated by inserting the transmembrane domain of the HIV-1 accessory peptide Vpu1-32, which forms helix bundles with characteristic opening states. We elucidated different amilorides as potential drugs to inhibit channel activity of Vpu.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 49

ER -

Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu _1-32 from HIV-1

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