Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope

Hsiang Yu Chung, Wei Cheng Kuo, Yu Hsiang Cheng, Che Hang Yu, Shih Hsuan Chia, Cheng Yung Lin, Jie Shin Chen, Huai Jen Tsai, Andrey B. Fedotov, Anatoly A. Ivanov, Aleksei M. Zheltikov, Chi Kuang Sun

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.

Original languageEnglish
Pages (from-to)31604-31614
Number of pages11
JournalOptics Express
Volume21
Issue number25
DOIs
StatePublished - 16 Dec 2013

Fingerprint

Dive into the research topics of 'Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope'. Together they form a unique fingerprint.

Cite this