Background removal in pump-probe microscopy

Subir Das, Bo Wei Ho, Fu Jen Kao*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


We have established a pump-probe microscopy with lock-in amplifier for the detection of spontaneous loss and stimulated gain. ATTO647N fluorescent is used for imaging. In order to separate the signal of interest from background, spontaneous loss is also detected, in addition to the stimulated gain. In this way, optical section images can be obtained with greatly reduced background, which is the origin of shot noise.

Original languageEnglish
Title of host publicationFrontiers in Optics, FiO 2017
PublisherOSA - The Optical Society
ISBN (Electronic)9781557528209
StatePublished - 2017
EventFrontiers in Optics, FiO 2017 - Washington, United States
Duration: 18 Sep 201721 Sep 2017

Publication series

NameOptics InfoBase Conference Papers
VolumePart F66-FiO 2017


ConferenceFrontiers in Optics, FiO 2017
Country/TerritoryUnited States


Dive into the research topics of 'Background removal in pump-probe microscopy'. Together they form a unique fingerprint.

Cite this