Background free imaging in stimulated emission fluorescence microscopy

Subir Das, I. Che Chen, Khalil Ur Rehman, Jung Lung Hsu, Guan Yu Zhuo, Fu Jen Kao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Recently, the pump-probe technique has found highly valued developments and applications in optical microscopy to study both labeled (fluorescence) and non-labeled (vibrational) for observation of cells and tissues. In this work, we use the mechanism of stimulated emission (SE) with dual frequency modulation to remove the large background signal that is originated from spontaneous emission. We have applied a high performance digital signal processing based lock-in detection for stimulated emission fluorescence microscopy. Critically, the pump beam is modulated at f 1 and the probe beam is at a different frequency, f 2, with the demodulation carried out at the sum of the two frequencies, (f 1 + f 2). In this way, the DC background is effectively removed. Note that the background is mainly attributed to the spontaneous emission of the pump beam, with modulated frequency f1.

Original languageEnglish
Article number125301
JournalJournal of Optics (United Kingdom)
Volume21
Issue number12
DOIs
StatePublished - 7 Nov 2019

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