Aurora-A phosphorylates hnRNPK and disrupts its interaction with p53

Kai Wei Hsueh, Shu Ling Fu, Chi Ying F. Huang, Chao Hsiung Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Amplification of Aurora-A, encoding a cell cycle-regulating kinase, has been reported in human cancers. Although Aurora-A is known to directly phosphorylate and down-regulate p53, the detailed mechanism remains unclear. Here we show that Aurora-A phosphorylates hnRNPK, a transcriptional coactivator of p53, on serine 379. This phosphorylation does not affect the post-transcriptional activity or cellular localization of hnRNPK, but disrupts its interaction with p53. Inverse correlation between Aurora-A activity and hnRNPK-p53 interaction was further demonstrated in DNA-damaged cells. Our results provide an alternative mechanism, whereby via phosphorylating hnRNPK Aurora-A participates in regulating p53 activity during DNA damage.

Original languageEnglish
Pages (from-to)2671-2675
Number of pages5
JournalFEBS Letters
Volume585
Issue number17
DOIs
StatePublished - 2 Sep 2011

Keywords

  • Aurora-A
  • hnRNPK
  • p53 Interaction

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