Attachment of stem cells on porous chitosan scaffold crosslinked by Na5P3O10

Hong Ru Lin, Ko Shao Chen*, Su Chen Chen, Chih Hung Lee, Shih Hwa Chiou, Ta Li Chang, Te Hsing Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In this study, porous chitosan scaffolds were prepared by freeze-dried method using Na5P3O10 as a crosslinking agent. The three-dimensional pore structure of the scaffold was interconnected with a mean pore size about 40 to 100 μm. The remained weight of crosslinked scaffold was about 76% after being exposed to PBS for 30 days. Mouse embryonic stem (E. S.) cells could grow on these crosslinked scaffolds. E. S. cells differentiated to other cells after 21 days of culturing on the scaffolds. The growth rate of E. S. cells was improved by post surface treatment of the scaffolds with collagen. However, there was no significant increase in growth rate of E. S. cells when scaffolds were surface treated with argon plasma. These porous chitosan scaffolds present a promising approach for tissue engineering applications.

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalMaterials Science and Engineering C
Volume27
Issue number2
DOIs
StatePublished - Mar 2007

Keywords

  • Cell culture
  • Chitosan
  • Scaffold
  • Stem cells

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