Abstract
In this study, porous chitosan scaffolds were prepared by freeze-dried method using Na5P3O10 as a crosslinking agent. The three-dimensional pore structure of the scaffold was interconnected with a mean pore size about 40 to 100 μm. The remained weight of crosslinked scaffold was about 76% after being exposed to PBS for 30 days. Mouse embryonic stem (E. S.) cells could grow on these crosslinked scaffolds. E. S. cells differentiated to other cells after 21 days of culturing on the scaffolds. The growth rate of E. S. cells was improved by post surface treatment of the scaffolds with collagen. However, there was no significant increase in growth rate of E. S. cells when scaffolds were surface treated with argon plasma. These porous chitosan scaffolds present a promising approach for tissue engineering applications.
Original language | English |
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Pages (from-to) | 280-284 |
Number of pages | 5 |
Journal | Materials Science and Engineering C |
Volume | 27 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2007 |
Keywords
- Cell culture
- Chitosan
- Scaffold
- Stem cells