Applications of surface enhanced spectroscopic techniques to biosystems

Gerald Steiner; Christian Kuhne; Beate Leupolt; Wolfang B. Fischer; Reiner Salzer

doi:10.1117/12.307072

Applications of surface enhanced spectroscopic techniques to biosystems

Gerald Steiner^*, Christian Kuhne, Beate Leupolt, Wolfang B. Fischer, Reiner Salzer

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

The potential of surface enhanced spectroscopy consists in the detection of very small but specific structures of biomaterials, Surface enhanced infrared absorption (SEIRA) yields fingerprint information on the biomaterials. Electronic interactions between individual groups in the molecule are detected by means of surface enhanced fluorescence (SEF). During the investigation of biomembranes with SEIRA a tenfold intensity enhancement could be obtained. Enhancement factors greater than 100 can be achieved with SEF. Enhancement is considerably influenced by the properties of the metal cluster structure. Biomembranes formed from vesicles containing the nicotinic acetylcholine receptor were spectroscopically characterized. The adsorption of the vesicles on Ge- and Ag surfaces was investigated. The metal cluster structure was optimized in order to obtain high intensity enhancement factors.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3256
DOIs	https://doi.org/10.1117/12.307072
State	Published - 1998
Event	Advances in Optical Biophysics - San Jose, CA, United States Duration: 25 Jan 1998 → 26 Jan 1998

Keywords

Biomembrane
Cluster
FTIR spectroscopy
Fluorescence
SEIRA
Surface enhanced spectroscopy

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title = "Applications of surface enhanced spectroscopic techniques to biosystems",

abstract = "The potential of surface enhanced spectroscopy consists in the detection of very small but specific structures of biomaterials, Surface enhanced infrared absorption (SEIRA) yields fingerprint information on the biomaterials. Electronic interactions between individual groups in the molecule are detected by means of surface enhanced fluorescence (SEF). During the investigation of biomembranes with SEIRA a tenfold intensity enhancement could be obtained. Enhancement factors greater than 100 can be achieved with SEF. Enhancement is considerably influenced by the properties of the metal cluster structure. Biomembranes formed from vesicles containing the nicotinic acetylcholine receptor were spectroscopically characterized. The adsorption of the vesicles on Ge- and Ag surfaces was investigated. The metal cluster structure was optimized in order to obtain high intensity enhancement factors.",

keywords = "Biomembrane, Cluster, FTIR spectroscopy, Fluorescence, SEIRA, Surface enhanced spectroscopy",

author = "Gerald Steiner and Christian Kuhne and Beate Leupolt and Fischer, {Wolfang B.} and Reiner Salzer",

year = "1998",

doi = "10.1117/12.307072",

language = "English",

volume = "3256",

pages = "106--112",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Advances in Optical Biophysics ; Conference date: 25-01-1998 Through 26-01-1998",

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TY - JOUR

T1 - Applications of surface enhanced spectroscopic techniques to biosystems

AU - Steiner, Gerald

AU - Kuhne, Christian

AU - Leupolt, Beate

AU - Fischer, Wolfang B.

AU - Salzer, Reiner

PY - 1998

Y1 - 1998

N2 - The potential of surface enhanced spectroscopy consists in the detection of very small but specific structures of biomaterials, Surface enhanced infrared absorption (SEIRA) yields fingerprint information on the biomaterials. Electronic interactions between individual groups in the molecule are detected by means of surface enhanced fluorescence (SEF). During the investigation of biomembranes with SEIRA a tenfold intensity enhancement could be obtained. Enhancement factors greater than 100 can be achieved with SEF. Enhancement is considerably influenced by the properties of the metal cluster structure. Biomembranes formed from vesicles containing the nicotinic acetylcholine receptor were spectroscopically characterized. The adsorption of the vesicles on Ge- and Ag surfaces was investigated. The metal cluster structure was optimized in order to obtain high intensity enhancement factors.

AB - The potential of surface enhanced spectroscopy consists in the detection of very small but specific structures of biomaterials, Surface enhanced infrared absorption (SEIRA) yields fingerprint information on the biomaterials. Electronic interactions between individual groups in the molecule are detected by means of surface enhanced fluorescence (SEF). During the investigation of biomembranes with SEIRA a tenfold intensity enhancement could be obtained. Enhancement factors greater than 100 can be achieved with SEF. Enhancement is considerably influenced by the properties of the metal cluster structure. Biomembranes formed from vesicles containing the nicotinic acetylcholine receptor were spectroscopically characterized. The adsorption of the vesicles on Ge- and Ag surfaces was investigated. The metal cluster structure was optimized in order to obtain high intensity enhancement factors.

KW - Biomembrane

KW - Cluster

KW - FTIR spectroscopy

KW - Fluorescence

KW - SEIRA

KW - Surface enhanced spectroscopy

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U2 - 10.1117/12.307072

DO - 10.1117/12.307072

M3 - Conference article

AN - SCOPUS:0032226279

SN - 0277-786X

VL - 3256

SP - 106

EP - 112

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Advances in Optical Biophysics

Y2 - 25 January 1998 through 26 January 1998

ER -

Applications of surface enhanced spectroscopic techniques to biosystems

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