An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus

Wen Hsin Chang; Sung Yi Yang; Yi Cheng Lin; Chih Hung Wang; Huey Ling You; Jiunn Jong Wu; Mel S. Lee; Gwo Bin Lee

doi:10.1109/NEMS.2016.7758321

An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus

Wen Hsin Chang, Sung Yi Yang, Yi Cheng Lin, Chih Hung Wang, Huey Ling You, Jiunn Jong Wu, Mel S. Lee, Gwo Bin Lee

Department of Biotechnology and Laboratory Science in Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.

Original language	English
Title of host publication	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	591-594
Number of pages	4
ISBN (Electronic)	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758321
State	Published - 28 Nov 2016
Event	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan Duration: 17 Apr 2016 → 20 Apr 2016

Publication series

Name	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Conference

Conference	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Country/Territory	Japan
City	Sendai
Period	17/04/16 → 20/04/16

Keywords

live hetero-bacteria
loop-mediated isothermal amplification
microfluidic
vancomycin resistant gene

Access to Document

10.1109/NEMS.2016.7758321

Cite this

Chang, W. H., Yang, S. Y., Lin, Y. C., Wang, C. H., You, H. L., Wu, J. J., Lee, M. S., & Lee, G. B. (2016). An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 591-594). Article 7758321 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758321

Chang, Wen Hsin ; Yang, Sung Yi ; Lin, Yi Cheng et al. / An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 591-594 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

@inproceedings{8857ea6ad52541ba823d5b7ede9f1281,

title = "An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus",

abstract = "This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.",

keywords = "live hetero-bacteria, loop-mediated isothermal amplification, microfluidic, vancomycin resistant gene",

author = "Chang, {Wen Hsin} and Yang, {Sung Yi} and Lin, {Yi Cheng} and Wang, {Chih Hung} and You, {Huey Ling} and Wu, {Jiunn Jong} and Lee, {Mel S.} and Lee, {Gwo Bin}",

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Chang, WH, Yang, SY, Lin, YC, Wang, CH, You, HL, Wu, JJ, Lee, MS & Lee, GB 2016, An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus. in 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758321, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., pp. 591-594, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 17/04/16. https://doi.org/10.1109/NEMS.2016.7758321

An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus. / Chang, Wen Hsin; Yang, Sung Yi; Lin, Yi Cheng et al.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 591-594 7758321 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus

AU - Chang, Wen Hsin

AU - Yang, Sung Yi

AU - Lin, Yi Cheng

AU - Wang, Chih Hung

AU - You, Huey Ling

AU - Wu, Jiunn Jong

AU - Lee, Mel S.

AU - Lee, Gwo Bin

PY - 2016/11/28

Y1 - 2016/11/28

N2 - This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.

AB - This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated microfluidic system including a microfluidic chip and a home-made control system was also demonstrated. The experimental results showed that the developed system can detect the vanA gene from 'live' Enterococcus but not 'dead' Enterococcus successfully with a detection limit of 10 colony formation units (CFU). The entire process including the sample pre-treatment process could be automated within 1 hour. This is the first time that an integrated microfluidic system was demonstrated to diagnose the vanA gene from live bacteria by using a molecular diagnosis approach. With its high sensitivity and accuracy, the proposed system may be promising to verify antibiotic resistance genes from live hetero-bacteria which cannot be achieved by using the existing diagnostic methods.

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M3 - Conference contribution

AN - SCOPUS:85007188839

T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

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BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

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Chang WH, Yang SY, Lin YC, Wang CH, You HL, Wu JJ et al. An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 591-594. 7758321. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). doi: 10.1109/NEMS.2016.7758321

An integrated microfluidic system for antibiotic resistance gene identification capable differentiating live and dead of vancomycin-resistant enterococcus

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