An integrated electrophoretic mobility control device with split design for signal improvement in liquid chromatography–electrospray ionization mass spectrometry analysis of aminoglycosides using a heptafluorobutyric acid containing mobile phase

Sih Hua Hung, Meng Ju Yu, Nan Hsuan Wang, Ren Yu Hsu, Guor Jien Wei, Guor Rong Her*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Electrophoretic mobility control (EMC) was used to alleviate the adverse effect of the ion-pairing agent heptafluorobutyric acid (HFBA) in the liquid chromatography–electrospray ionization mass spectrometry (LC-ESI-MS) analysis of aminoglycosides. Aminoglycosides separated by LC were directed to a connecting column before their detection via ESI. Applying an electric field across the connecting column caused the positively charged aminoglycosides to migrate toward the mass spectrometer whereas the HFBA anions remained in the junction reservoir, thus alleviating the ion suppression caused by HFBA. To accommodate the flow rate of a narrow-bore column, minimize the effect of electrophoretic mobility on separation, and facilitate the operation, an integrated EMC device with a split design was fabricated. With the proposed EMC device, the signals of aminoglycosides were enhanced by a factor of 5–85 without affecting the separation efficiency or elution order. For the analysis of aminoglycosides in bovine milk, the proposed approach demonstrates a sensitivity that is at least 10 times below the maximum residue limits set by most countries.

Original languageEnglish
Pages (from-to)156-163
Number of pages8
JournalAnalytica Chimica Acta
Volume933
DOIs
StatePublished - 24 Aug 2016

Keywords

  • Aminoglycoside
  • Electrophoretic mobility control
  • Heptafluorobutyric acid
  • Ion pairing agent

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