An alleviative effect of Lonicerae japonicae flos water extract against liver fibrogenesis in vitro and in vivo

Yi Ling Lin, Yi Hsieng Samuel Wu, Ming Yuan Chao, Deng Jye Yang, Cheng Wei Liu, Jung Kai Tseng*, Yi Chen Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Lonicerae japonicae (L. japonicae) flos is a medical and food homology herb. This study investigated the phenolic acid and flavonoid contents in L. japonicae flos water extract solution (LJWES) and the preventive effects of LJWES against liver fibrogenesis via FL83B cells and rats. LJWES contains many polyphenols, such as chlorogenic acid, morin, and epicatechin. LJWES increased cell viability and decreased cytotoxicity in thioacetamide (TAA)-treated FL83B cells (75 mM) (p <.05). LJWES decreased (p <.05) gene expressions of Tnf-α, Tnfr1, Bax, and cytochrome c but upregulated Bcl-2 and Bcl-xl in TAA-treated cells; meanwhile, increased protein levels of P53, cleaved caspase 3, and cleaved caspase 9 in TAA treated cells were downregulated (p <.05) by LJWES supplementation. In vivo, results indicated that TAA treatment increased serum liver damage indices (alanine aminotransferase [ALT] and alkaline phosphatase [ALP]) and cytokines (interleukin-6 and transforming growth factor-β1) levels and impaired liver antioxidant capacities (increased thiobarbituric acid reactive substance value but decreased catalase/glutathione peroxidase activities) in rats (p <.05) while LJWES supplementation amended (p <.05) them. Liver fibrosis scores, collagen deposition, and alpha-smooth muscle actin deposition in TAA-treated rats were also decreased by LJWES supplementation (p <.05). To sum up, LJWES could be a potential hepatoprotective agent against liver fibrogenesis by enhancing antioxidant ability, downregulating inflammation in livers, and reducing apoptosis in hepatocytes.

Original languageEnglish
Pages (from-to)2881-2892
Number of pages12
JournalEnvironmental Toxicology
Issue number5
StatePublished - May 2024


  • Lonicera japonicae flos
  • anti-inflammation
  • antiapoptosis
  • antioxidation
  • liver fibrogenesis


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