Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity

Fu Hsien Yu; Kuo Jung Huang; Chin Tien Wang

doi:10.1371/journal.pone.0262477

Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity

Fu Hsien Yu, Kuo Jung Huang, Chin Tien Wang^*

^*Corresponding author for this work

Institute of Clinical Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A transframe region within HIV-1 Gag-Pol (referred to as p6*or p6pol), directly linked to the protease (PR) N-terminus, plays a pivotal role in modulating PR activation. To identify specific p6*residues involved in PR activation, we created a series of p6*mutants by making substitutions for conserved p6*residues. Our results indicate that some p6*mutants were defective in terms of virus infectivity, despite displaying a wild-type virus particle processing pattern. Mutations at p6*F8 reduced virus infectivity associated with insufficient virus processing, due in part to impaired PR maturation and RT packaging. Our data strongly suggest that conserved Phe (F) residues at position 8 of p6*are involved in the PR maturation process.

Original language	English
Article number	e0262477
Journal	PLoS ONE
Volume	17
Issue number	1 January
DOIs	https://doi.org/10.1371/journal.pone.0262477
State	Published - Jan 2022

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abstract = "A transframe region within HIV-1 Gag-Pol (referred to as p6*or p6pol), directly linked to the protease (PR) N-terminus, plays a pivotal role in modulating PR activation. To identify specific p6*residues involved in PR activation, we created a series of p6*mutants by making substitutions for conserved p6*residues. Our results indicate that some p6*mutants were defective in terms of virus infectivity, despite displaying a wild-type virus particle processing pattern. Mutations at p6*F8 reduced virus infectivity associated with insufficient virus processing, due in part to impaired PR maturation and RT packaging. Our data strongly suggest that conserved Phe (F) residues at position 8 of p6*are involved in the PR maturation process. ",

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Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity

Abstract

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