Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

Ming Hui Yang; Yuan Han Yang; Chi Yu Lu; Shiang Bin Jong; Li Jhen Chen; Yu Fen Lin; Shyh Jong Wu; Pei Yu Chu; Tze Wen Chung; Yu Chang Tyan

doi:10.1016/j.jprot.2012.04.017

Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

Ming Hui Yang, Yuan Han Yang, Chi Yu Lu, Shiang Bin Jong, Li Jhen Chen, Yu Fen Lin, Shyh Jong Wu, Pei Yu Chu, Tze Wen Chung, Yu Chang Tyan^*

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.

Original language	English
Pages (from-to)	3617-3629
Number of pages	13
Journal	Journal of Proteomics
Volume	75
Issue number	12
DOIs	https://doi.org/10.1016/j.jprot.2012.04.017
State	Published - 27 Jun 2012

Keywords

ADNP
Alzheimer's disease
Biomarker
Mass spectrometry
Proteomics

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10.1016/j.jprot.2012.04.017

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@article{d182b5593b1040c99a8aed2accf551bc,

title = "Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease",

abstract = "Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.",

keywords = "ADNP, Alzheimer's disease, Biomarker, Mass spectrometry, Proteomics",

author = "Yang, \{Ming Hui\} and Yang, \{Yuan Han\} and Lu, \{Chi Yu\} and Jong, \{Shiang Bin\} and Chen, \{Li Jhen\} and Lin, \{Yu Fen\} and Wu, \{Shyh Jong\} and Chu, \{Pei Yu\} and Chung, \{Tze Wen\} and Tyan, \{Yu Chang\}",

note = "Funding Information: We are thankful to S. Sheldon MT (ASCP) of Oklahoma University Medical Center Edmond (USA) for fruitful discussions. The authors thank National Cheng-Kung University Proteomics Research Core Laboratory for the assistance in protein identification. This work was supported by research grants NSC-099-2811-E-22-002 and NSC-100-2320-B-37-007-MY3 from the National Science Council, and NSYSUKMU 101-015 from NSYSU-KMU Joint Research Project, Taiwan, Republic of China. ",

year = "2012",

month = jun,

day = "27",

doi = "10.1016/j.jprot.2012.04.017",

language = "English",

volume = "75",

pages = "3617--3629",

journal = "Journal of Proteomics",

issn = "1874-3919",

publisher = "Elsevier B.V.",

number = "12",

}

TY - JOUR

T1 - Activity-dependent neuroprotector homeobox protein

T2 - A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

AU - Yang, Ming Hui

AU - Yang, Yuan Han

AU - Lu, Chi Yu

AU - Jong, Shiang Bin

AU - Chen, Li Jhen

AU - Lin, Yu Fen

AU - Wu, Shyh Jong

AU - Chu, Pei Yu

AU - Chung, Tze Wen

AU - Tyan, Yu Chang

N1 - Funding Information: We are thankful to S. Sheldon MT (ASCP) of Oklahoma University Medical Center Edmond (USA) for fruitful discussions. The authors thank National Cheng-Kung University Proteomics Research Core Laboratory for the assistance in protein identification. This work was supported by research grants NSC-099-2811-E-22-002 and NSC-100-2320-B-37-007-MY3 from the National Science Council, and NSYSUKMU 101-015 from NSYSU-KMU Joint Research Project, Taiwan, Republic of China.

PY - 2012/6/27

Y1 - 2012/6/27

N2 - Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.

AB - Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.

KW - ADNP

KW - Alzheimer's disease

KW - Biomarker

KW - Mass spectrometry

KW - Proteomics

UR - https://www.scopus.com/pages/publications/84862209675

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M3 - Article

C2 - 22554909

AN - SCOPUS:84862209675

SN - 1874-3919

VL - 75

SP - 3617

EP - 3629

JO - Journal of Proteomics

JF - Journal of Proteomics

IS - 12

ER -

Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

Abstract

Keywords

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