Activation of NK cell cytotoxicity by the natural compound 2,3-butanediol

Hsin Chih Lai, Chih Jung Chang, Chun Hung Yang, Ya Jing Hsu, Chang Chieh Chen, Chuan Sheng Lin, Yu Huan Tsai, Tsung Teng Huang, David M. Ojcius, Ying Huang Tsai, Chia Chen Lu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD enhances NK cell cytotoxic activity in human pNK cells and NK92 cells. Treatment of NK cells with 2,3-BTD increased perforin expression in a dose-dependent manner. This was accompanied by elevated JNK and ERK1/2 MAPK activities and enhanced expression of NKG2D/ NCRs, upstream signaling molecules of the MAPK pathways. The 2,3-BTD effect was inhibited by pretreatment with inhibitors of JNK (SP) or ERK1/2 (PD) or by depleting NKG2D/NCRs or JNK1 or ERK2 with siRNA. These results indicate that 2,3-BTD activates NK cell cytotoxicity by NKG2D/NCR pathways and represent the first report of the 2,3-BTD effect on activation of innate immunity cells.

Original languageEnglish
Pages (from-to)807-814
Number of pages8
JournalJournal of Leukocyte Biology
Volume92
Issue number4
DOIs
StatePublished - Oct 2012

Keywords

  • NCR
  • NKG2D
  • Perforin
  • Resveratrol

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