Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

Ching Hao Li; Chen Chen Lee; Yu Wen Cheng; Hwe Ann Juang; Jaw Jou Kang

doi:10.1016/j.toxlet.2004.03.003

Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

Ching Hao Li, Chen Chen Lee, Yu Wen Cheng, Hwe Ann Juang, Jaw Jou Kang^*

^*Corresponding author for this work

Institute of Food Safety and Health Risk Assessment

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca²⁺ influx and hence elevation of intracellular Ca²⁺ concentration and NO production. The effect can be inhibited by Ca²⁺ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl₂ and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca²⁺-dependent manner.

Original language	English
Pages (from-to)	367-374
Number of pages	8
Journal	Toxicology Letters
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/j.toxlet.2004.03.003
State	Published - 15 Jul 2004

Keywords

Ca
Endothelium
HUVECs
NO
PAHs
Polycyclic aromatic hydrocarbons
eNOS
endothelial nitric oxide synthase
human umbilical vein endothelial cells
polycyclic aromatic hydrocarbons

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10.1016/j.toxlet.2004.03.003

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@article{36fd08d3ab0f4fccbd754ed578fd908d,

title = "Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons",

abstract = "PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.",

keywords = "Ca, Endothelium, HUVECs, NO, PAHs, Polycyclic aromatic hydrocarbons, eNOS, endothelial nitric oxide synthase, human umbilical vein endothelial cells, polycyclic aromatic hydrocarbons",

author = "Li, {Ching Hao} and Lee, {Chen Chen} and Cheng, {Yu Wen} and Juang, {Hwe Ann} and Kang, {Jaw Jou}",

note = "Funding Information: The research is supported by the grant from National Science Council, Republic of China.",

year = "2004",

month = jul,

day = "15",

doi = "10.1016/j.toxlet.2004.03.003",

language = "English",

volume = "151",

pages = "367--374",

journal = "Toxicology Letters",

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T1 - Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

AU - Li, Ching Hao

AU - Lee, Chen Chen

AU - Cheng, Yu Wen

AU - Juang, Hwe Ann

AU - Kang, Jaw Jou

N1 - Funding Information: The research is supported by the grant from National Science Council, Republic of China.

PY - 2004/7/15

Y1 - 2004/7/15

N2 - PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

AB - PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

KW - Ca

KW - Endothelium

KW - HUVECs

KW - NO

KW - PAHs

KW - Polycyclic aromatic hydrocarbons

KW - eNOS

KW - endothelial nitric oxide synthase

KW - human umbilical vein endothelial cells

KW - polycyclic aromatic hydrocarbons

UR - http://www.scopus.com/inward/record.url?scp=2942616784&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2004.03.003

DO - 10.1016/j.toxlet.2004.03.003

M3 - Article

C2 - 15183461

AN - SCOPUS:2942616784

SN - 0378-4274

VL - 151

SP - 367

EP - 374

JO - Toxicology Letters

JF - Toxicology Letters

IS - 2

ER -

Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

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Keywords

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