AC electrokinetics assisted impedance biosensors for rapid bacteria detection

Yi Tse Ho*, Chun Liang Pan, Jeng-Tzong Sheu, Yu Kuo Wang, Tung-Kung Wu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Early detection of living bacteria attracts a lot of attention in infection diseases applications, especially at low concentration. Nowadays, the detection of specific bacteria faces challenges including enhancement of trapping efficiency, and increase of sensor sensitivity and specificity. In this study, we improved the trapping efficiency and increased sensor sensitivity and specificity by using AC electrokinetics and selective modification of antibody on gold electrode in a new structure of gold electrode design. COMSOL simulations of AC electrokinetics include dielectrophoresis (DEP), and AC electroosmosis (ACEO) were performed on different electrode designs and bias configurations. The simulation results were consistent with the fluorescence observations showing that the bacteria were trapping selectively on gold electrodes. The impedance measurements for Vibrio parahaemolyticus (Vp) detection at 105 cfu/ml with AC elctrokinetics showed a nearly 90% change in normalized impedance change (NIC).

Original languageEnglish
Title of host publication2012 IEEE Biomedical Circuits and Systems Conference
Subtitle of host publicationIntelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012 - Conference Publications
Pages49-52
Number of pages4
DOIs
StatePublished - 1 Dec 2012
Event2012 IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012 - Hsinchu, Taiwan
Duration: 28 Nov 201230 Nov 2012

Publication series

Name2012 IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012 - Conference Publications

Conference

Conference2012 IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Electronics and Systems for Better Life and Better Environment, BioCAS 2012
Country/TerritoryTaiwan
CityHsinchu
Period28/11/1230/11/12

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