A two photon fluorescent probe for highly selective detection and endogenous imaging of hydrogen sulfide

Wan Yu Yang, Kathiresan Anusuyadevi, Ping Hsuan Lu, Natesan Thirumalaivasan, Wen Hsuan Lin, Sivan Velmathi, Shu Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Hydrogen sulfide (H2S), one of redox-active sulfur species, is known as a signaling molecule and an antioxidant in biological tissues to maintain cellular functions. The development of selective and sensitive H2S detection is important to understand the role of H2S in vivo. Herein, a new two-photon probe NNE was developed to detect hydrogen sulfide using 6-acetyl-N-methyl-2-naphthylamine with an attachment of 7-nitrobenzo-oxadiazole. The probe NNE exhibits high selectivity towards hydrogen sulfide over other anions. Nucleophilic substitution of H2S leads to a turn-on response with 28-fold enhancement in quantum yield (from 0.004 to 0.117). NNE shows a high sensitivity towards hydrogen sulfide with an extremely low detection limit at 6.8 nM. Furthermore, the probe NNE exhibits two-photon excited fluorescence, making it a suitable probe for monitoring H2S distribution in live cells and tissues without background fluorescence interference.

Original languageEnglish
Article number121043
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume273
DOIs
StatePublished - 15 May 2022

Keywords

  • Endogenous cell imaging
  • Fluorescent probe
  • HS
  • Two-photon

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