A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells

Chao Chin Hu; Jau Tien Lin; Shih Chuan Liu; Deng Jye Yang

doi:10.38212/2224-6614.2406

A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells

Chao Chin Hu, Jau Tien Lin, Shih Chuan Liu, Deng Jye Yang^*

^*Corresponding author for this work

Institute of Food Safety and Health Risk Assessment

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A spirostanol glycoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl -(1→2)-O-[β-D-glucopyranosyl -(1→4)]-β-D-glucopyranoside, was isolated from wild yam (Dioscorea villosa) extract with reversed-phase preparative HPLC. This was the first time found in yams (Dioscorea species). The structure was identified by LC-MS, ¹H- and ¹³C-NMR. Cytostatic activities of the isolated saponin on Hep G2, HEK293 and MCF7 cells were estimated. Growth of these cells was inhibited as higher concentration of the compound was used. The IC₅₀ values of the isolated spirostanol glycoside and its aglycon, diosgenin, were 9.02 and 23.91 μM for Hep G2 cells, 13.21 and 27.31 μM for HEK293 cells, and 16.74 and 35.38 μM for MCF7 cells, respectively. The isolated compound had significantly higher active intensity than diosgenin.

Original language	English
Pages (from-to)	310-315
Number of pages	6
Journal	Journal of Food and Drug Analysis
Volume	15
Issue number	3
DOIs	https://doi.org/10.38212/2224-6614.2406
State	Published - Sep 2007

Keywords

Cytostatic activity
Dioscorea villosa
Diosgenin
HEK293 cells
Hep G2 cells
Isolation
MCF7 cells
SpirostanolGlycosid
Yam

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.38212/2224-6614.2406

Cite this

@article{9be95dc4c6c349b89d0ca809d93ebe2d,

title = "A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells",

abstract = "A spirostanol glycoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl -(1→2)-O-[β-D-glucopyranosyl -(1→4)]-β-D-glucopyranoside, was isolated from wild yam (Dioscorea villosa) extract with reversed-phase preparative HPLC. This was the first time found in yams (Dioscorea species). The structure was identified by LC-MS, 1H- and 13C-NMR. Cytostatic activities of the isolated saponin on Hep G2, HEK293 and MCF7 cells were estimated. Growth of these cells was inhibited as higher concentration of the compound was used. The IC50 values of the isolated spirostanol glycoside and its aglycon, diosgenin, were 9.02 and 23.91 μM for Hep G2 cells, 13.21 and 27.31 μM for HEK293 cells, and 16.74 and 35.38 μM for MCF7 cells, respectively. The isolated compound had significantly higher active intensity than diosgenin.",

keywords = "Cytostatic activity, Dioscorea villosa, Diosgenin, HEK293 cells, Hep G2 cells, Isolation, MCF7 cells, SpirostanolGlycosid, Yam",

author = "Hu, {Chao Chin} and Lin, {Jau Tien} and Liu, {Shih Chuan} and Yang, {Deng Jye}",

year = "2007",

month = sep,

doi = "10.38212/2224-6614.2406",

language = "English",

volume = "15",

pages = "310--315",

journal = "Journal of Food and Drug Analysis",

issn = "1021-9498",

publisher = "National Laboratories of Foods and Drugs",

number = "3",

}

TY - JOUR

T1 - A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells

AU - Hu, Chao Chin

AU - Lin, Jau Tien

AU - Liu, Shih Chuan

AU - Yang, Deng Jye

PY - 2007/9

Y1 - 2007/9

N2 - A spirostanol glycoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl -(1→2)-O-[β-D-glucopyranosyl -(1→4)]-β-D-glucopyranoside, was isolated from wild yam (Dioscorea villosa) extract with reversed-phase preparative HPLC. This was the first time found in yams (Dioscorea species). The structure was identified by LC-MS, 1H- and 13C-NMR. Cytostatic activities of the isolated saponin on Hep G2, HEK293 and MCF7 cells were estimated. Growth of these cells was inhibited as higher concentration of the compound was used. The IC50 values of the isolated spirostanol glycoside and its aglycon, diosgenin, were 9.02 and 23.91 μM for Hep G2 cells, 13.21 and 27.31 μM for HEK293 cells, and 16.74 and 35.38 μM for MCF7 cells, respectively. The isolated compound had significantly higher active intensity than diosgenin.

AB - A spirostanol glycoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl -(1→2)-O-[β-D-glucopyranosyl -(1→4)]-β-D-glucopyranoside, was isolated from wild yam (Dioscorea villosa) extract with reversed-phase preparative HPLC. This was the first time found in yams (Dioscorea species). The structure was identified by LC-MS, 1H- and 13C-NMR. Cytostatic activities of the isolated saponin on Hep G2, HEK293 and MCF7 cells were estimated. Growth of these cells was inhibited as higher concentration of the compound was used. The IC50 values of the isolated spirostanol glycoside and its aglycon, diosgenin, were 9.02 and 23.91 μM for Hep G2 cells, 13.21 and 27.31 μM for HEK293 cells, and 16.74 and 35.38 μM for MCF7 cells, respectively. The isolated compound had significantly higher active intensity than diosgenin.

KW - Cytostatic activity

KW - Dioscorea villosa

KW - Diosgenin

KW - HEK293 cells

KW - Hep G2 cells

KW - Isolation

KW - MCF7 cells

KW - SpirostanolGlycosid

KW - Yam

UR - http://www.scopus.com/inward/record.url?scp=36448983224&partnerID=8YFLogxK

U2 - 10.38212/2224-6614.2406

DO - 10.38212/2224-6614.2406

M3 - Article

AN - SCOPUS:36448983224

SN - 1021-9498

VL - 15

SP - 310

EP - 315

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

IS - 3

ER -

A spirostanol glycoside from wild yam (Diosccorea villosa) extract and its cytostatic activity on three cancer cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this