A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao; Chiu Min Cheng; Kuo Hsiang Chuang; Chih Hung Chuang; Shey-Cherng Tzou; Ta Chun Cheng; Yuan Chin Hsieh; Kuang-Wen Liao; Yun-Ming Wang; Long Sen Chang; Steve R. Roffler; Fang Ming Chen; Tian Lu Cheng

doi:10.1016/j.ab.2012.08.001

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao, Chiu Min Cheng, Kuo Hsiang Chuang, Chih Hung Chuang, Shey-Cherng Tzou, Ta Chun Cheng, Yuan Chin Hsieh, Kuang-Wen Liao, Yun-Ming Wang, Long Sen Chang, Steve R. Roffler, Fang Ming Chen^*, Tian Lu Cheng

^*Corresponding author for this work

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R ² = 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Analytical Biochemistry
Volume	431
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2012.08.001
State	Published - 1 Dec 2012

Keywords

14-Epitope tag protein ladder
Anti-epitope tag antibodies
Epitope tags
Protein molecular estimation
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Western blot analysis

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10.1016/j.ab.2012.08.001

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title = "A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis",

abstract = " We designed a protein ladder (hereafter referred to as {"}Mega- tag{"}) that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R 2 = 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.",

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doi = "10.1016/j.ab.2012.08.001",

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AU - Kao, Chien Han

AU - Cheng, Chiu Min

AU - Chuang, Kuo Hsiang

AU - Chuang, Chih Hung

AU - Tzou, Shey-Cherng

AU - Cheng, Ta Chun

AU - Hsieh, Yuan Chin

AU - Liao, Kuang-Wen

AU - Wang, Yun-Ming

AU - Chang, Long Sen

AU - Roffler, Steve R.

AU - Chen, Fang Ming

AU - Cheng, Tian Lu

PY - 2012/12/1

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AB - We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R 2 = 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

KW - 14-Epitope tag protein ladder

KW - Anti-epitope tag antibodies

KW - Epitope tags

KW - Protein molecular estimation

KW - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

KW - Western blot analysis

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SP - 1

EP - 3

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Abstract

Keywords

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