A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao, Chiu Min Cheng, Kuo Hsiang Chuang, Chih Hung Chuang, Shey-Cherng Tzou, Ta Chun Cheng, Yuan Chin Hsieh, Kuang-Wen Liao, Yun-Ming Wang, Long Sen Chang, Steve R. Roffler, Fang Ming Chen*, Tian Lu Cheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R 2 = 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalAnalytical Biochemistry
Issue number1
StatePublished - 1 Dec 2012


  • 14-Epitope tag protein ladder
  • Anti-epitope tag antibodies
  • Epitope tags
  • Protein molecular estimation
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
  • Western blot analysis


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