A rapid SNAP-tag fluorogenic probe based on an environment-sensitive fluorophore for no-wash live cell imaging

Tao Kai Liu, Pei Ying Hsieh, Yu De Zhuang, Chi Yang Hsia, Chi Ling Huang, Hsiu Ping Lai, Hung Sheung Lin, I. Chia Chen, Hsin-Yun Hsu*, Kui Thong Tan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

(Figure Presented). One major limitation of labeling proteins with synthetic fluorophores is the high fluorescence background, which necessitates extensive washing steps to remove unreacted fluorophores. In this paper, we describe a novel fluorogenic probe based on an environment-sensitive fluorophore for labeling with SNAP-tag proteins. The probe exhibits dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag. The major advantages of our fluorogenic probe are the dramatic fluorescence turn-on, ease of synthesis, high selectivity, and rapid labeling with SNAP-tag. No-wash labeling of both intracellular and cell surface proteins was successfully achieved in living cells, and the localization of these proteins was specifically visualized.

Original languageEnglish
Pages (from-to)2359-2365
Number of pages7
JournalACS Chemical Biology
Volume9
Issue number10
DOIs
StatePublished - 8 Aug 2014

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