A Programmable Bio-Chip with Adaptive Pattern-Control Micro-Electrode-Dot-Array

Yun Sheng Chan; Chen Yi Lee

doi:10.1109/TCSII.2022.3184749

A Programmable Bio-Chip with Adaptive Pattern-Control Micro-Electrode-Dot-Array

Yun Sheng Chan, Chen Yi Lee

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A digital lab-on-CMOS chip is presented for nucleic acid amplification test. This biochip consists of programmable microelectrode dot array and digital timing controller. With novel circuit design in each microelectrode, microfluidic operations with velocity of 0.6mm/s, temperature profile with maximum change rate of 4.5℃/s and location sensing in μsecond-level can be achieved. As a result, test samples can be located first, followed by target operations to meet the requirements of specific medical tests with less reagent consumption and test time/cost. Furthermore bio-protocols can be derived to ensure both replicability and reliability in various medical tests. Preliminary results in loop-mediated isothermal amplification (LAMP) and PCR thermal cycle tests demonstrate the feasibility of our proposal with less test time of <40 min and <1 min respectively, making our proposal very suitable for mobile healthcare applications.

Original language	English
Pages (from-to)	1
Number of pages	1
Journal	IEEE Transactions on Circuits and Systems I: Regular Papers
DOIs	https://doi.org/10.1109/TCSII.2022.3184749
State	Accepted/In press - 2022

Keywords

bio-protocol
CMOS technology
digital microfluidics biochip (DMFB)
electrowetting-on-dielectric (EWOD)
Heating systems
microelectrode dot array (MEDA)
Microelectrodes
Microfluidics
programmable biochip
Sensors
Temperature measurement
Temperature sensors

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10.1109/TCSII.2022.3184749

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title = "A Programmable Bio-Chip with Adaptive Pattern-Control Micro-Electrode-Dot-Array",

abstract = "A digital lab-on-CMOS chip is presented for nucleic acid amplification test. This biochip consists of programmable microelectrode dot array and digital timing controller. With novel circuit design in each microelectrode, microfluidic operations with velocity of 0.6mm/s, temperature profile with maximum change rate of 4.5℃/s and location sensing in μsecond-level can be achieved. As a result, test samples can be located first, followed by target operations to meet the requirements of specific medical tests with less reagent consumption and test time/cost. Furthermore bio-protocols can be derived to ensure both replicability and reliability in various medical tests. Preliminary results in loop-mediated isothermal amplification (LAMP) and PCR thermal cycle tests demonstrate the feasibility of our proposal with less test time of <40 min and <1 min respectively, making our proposal very suitable for mobile healthcare applications.",

keywords = "bio-protocol, CMOS technology, digital microfluidics biochip (DMFB), electrowetting-on-dielectric (EWOD), Heating systems, microelectrode dot array (MEDA), Microelectrodes, Microfluidics, programmable biochip, Sensors, Temperature measurement, Temperature sensors",

author = "Chan, {Yun Sheng} and Lee, {Chen Yi}",

note = "Publisher Copyright: IEEE",

year = "2022",

doi = "10.1109/TCSII.2022.3184749",

language = "English",

pages = "1",

journal = "IEEE Transactions on Circuits and Systems I: Regular Papers",

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publisher = "Institute of Electrical and Electronics Engineers Inc.",

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AU - Chan, Yun Sheng

AU - Lee, Chen Yi

N1 - Publisher Copyright: IEEE

PY - 2022

Y1 - 2022

N2 - A digital lab-on-CMOS chip is presented for nucleic acid amplification test. This biochip consists of programmable microelectrode dot array and digital timing controller. With novel circuit design in each microelectrode, microfluidic operations with velocity of 0.6mm/s, temperature profile with maximum change rate of 4.5℃/s and location sensing in μsecond-level can be achieved. As a result, test samples can be located first, followed by target operations to meet the requirements of specific medical tests with less reagent consumption and test time/cost. Furthermore bio-protocols can be derived to ensure both replicability and reliability in various medical tests. Preliminary results in loop-mediated isothermal amplification (LAMP) and PCR thermal cycle tests demonstrate the feasibility of our proposal with less test time of <40 min and <1 min respectively, making our proposal very suitable for mobile healthcare applications.

AB - A digital lab-on-CMOS chip is presented for nucleic acid amplification test. This biochip consists of programmable microelectrode dot array and digital timing controller. With novel circuit design in each microelectrode, microfluidic operations with velocity of 0.6mm/s, temperature profile with maximum change rate of 4.5℃/s and location sensing in μsecond-level can be achieved. As a result, test samples can be located first, followed by target operations to meet the requirements of specific medical tests with less reagent consumption and test time/cost. Furthermore bio-protocols can be derived to ensure both replicability and reliability in various medical tests. Preliminary results in loop-mediated isothermal amplification (LAMP) and PCR thermal cycle tests demonstrate the feasibility of our proposal with less test time of <40 min and <1 min respectively, making our proposal very suitable for mobile healthcare applications.

KW - bio-protocol

KW - CMOS technology

KW - digital microfluidics biochip (DMFB)

KW - electrowetting-on-dielectric (EWOD)

KW - Heating systems

KW - microelectrode dot array (MEDA)

KW - Microelectrodes

KW - Microfluidics

KW - programmable biochip

KW - Sensors

KW - Temperature measurement

KW - Temperature sensors

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JO - IEEE Transactions on Circuits and Systems I: Regular Papers

JF - IEEE Transactions on Circuits and Systems I: Regular Papers

ER -

A Programmable Bio-Chip with Adaptive Pattern-Control Micro-Electrode-Dot-Array

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Keywords

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