A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Yuan Pin Lo; Narayanasamy Nivetha; Sivan Velmathi; Shu Pao Wu

doi:10.1016/j.ymeth.2023.12.004

A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Yuan Pin Lo, Narayanasamy Nivetha, Sivan Velmathi, Shu Pao Wu^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10^-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

Original language	English
Pages (from-to)	10-18
Number of pages	9
Journal	Methods
Volume	222
DOIs	https://doi.org/10.1016/j.ymeth.2023.12.004
State	Published - Feb 2024

Keywords

Fluorescent probe
Near-infrared
Ovarian cancer cells
Stokes shift
β-Galactosidase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ymeth.2023.12.004

Cite this

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title = "A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals",

abstract = "β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.",

keywords = "Fluorescent probe, Near-infrared, Ovarian cancer cells, Stokes shift, β-Galactosidase",

author = "Lo, {Yuan Pin} and Narayanasamy Nivetha and Sivan Velmathi and Wu, {Shu Pao}",

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doi = "10.1016/j.ymeth.2023.12.004",

language = "English",

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T1 - A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

AU - Lo, Yuan Pin

AU - Nivetha, Narayanasamy

AU - Velmathi, Sivan

AU - Wu, Shu Pao

PY - 2024/2

Y1 - 2024/2

N2 - β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

AB - β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

KW - Fluorescent probe

KW - Near-infrared

KW - Ovarian cancer cells

KW - Stokes shift

KW - β-Galactosidase

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DO - 10.1016/j.ymeth.2023.12.004

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AN - SCOPUS:85181679446

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JO - Methods

JF - Methods

ER -

A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Abstract

Keywords

UN SDGs

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