A near-infrared fluorescent probe with a substantial Stokes shift designed for the detection and imaging of β-galactosidase within living cells and animals

Yuan Pin Lo, Narayanasamy Nivetha, Sivan Velmathi, Shu Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

β-Galactosidase serves as a pivotal biomarker for both cancer and cellular aging. The advancement of fluorescent sensors for tracking β-galactosidase activity is imperative in the realm of cancer diagnosis. We have designed a near-infrared fluorescent probe (PTA-gal) for the detection of β-galactosidase in living systems with large Stokes shifts. PTA-gal exhibits remarkable sensitivity and selectivity in detecting β-galactosidase, producing near-infrared fluorescent signals with a remarkably low detection limit (2.2 × 10-5 U/mL) and a high quantum yield (0.30). Moreover, PTA-gal demonstrates biocompatibility and can effectively detect β-galactosidase in cancer cells as well as within living animals.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalMethods
Volume222
DOIs
StatePublished - Feb 2024

Keywords

  • Fluorescent probe
  • Near-infrared
  • Ovarian cancer cells
  • Stokes shift
  • β-Galactosidase

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