A nano-molar fluorescent turn-on probe for copper(II) detection in living cells

Ling Fang Wei; Chong Yan Chen; Chia Kai Lai; Natesan Thirumalaivasan; Shu-Pao Wu

doi:10.1016/j.ymeth.2019.04.023

A nano-molar fluorescent turn-on probe for copper(II) detection in living cells

Ling Fang Wei, Chong Yan Chen, Chia Kai Lai, Natesan Thirumalaivasan, Shu-Pao Wu^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The development of fluorescent probes to detect trace metal ions in biological samples has been in great need. Herein, a fluorescence turn-on sensor (PHC) was designed for highly selective detection of Cu²⁺ ions. The probe PHC shows weak fluorescence due to imine isomerization. With Cu²⁺, a significant blue emission due to Cu²⁺-induced oxidation of imine to a carboxylate group is observed. The turn-on process is observed with a 63-fold increase of fluorescence quantum yield (from 0.004 to 0.252). The emission intensity has a good linear relation at Cu²⁺ concentrations of 0–40 μM. The detection limit is estimated as 8 nM (S/N = 3). The maximum emission change induced by Cu²⁺ is found in the pH range of 6.5–8.0. The probe PHC can be applied in detecting Cu²⁺ in living cells monitored by confocal fluorescence microscopy imaging.

Original language	English
Pages (from-to)	18-23
Number of pages	6
Journal	Methods
Volume	168
DOIs	https://doi.org/10.1016/j.ymeth.2019.04.023
State	Published - 15 Sep 2019

Keywords

Bioimaging
Copper(II)
Coumarin
Fluorescence turn-on

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10.1016/j.ymeth.2019.04.023

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@article{933c84194a6949eca60b826ecad15065,

title = "A nano-molar fluorescent turn-on probe for copper(II) detection in living cells",

abstract = "The development of fluorescent probes to detect trace metal ions in biological samples has been in great need. Herein, a fluorescence turn-on sensor (PHC) was designed for highly selective detection of Cu2+ ions. The probe PHC shows weak fluorescence due to imine isomerization. With Cu2+, a significant blue emission due to Cu2+-induced oxidation of imine to a carboxylate group is observed. The turn-on process is observed with a 63-fold increase of fluorescence quantum yield (from 0.004 to 0.252). The emission intensity has a good linear relation at Cu2+ concentrations of 0–40 μM. The detection limit is estimated as 8 nM (S/N = 3). The maximum emission change induced by Cu2+ is found in the pH range of 6.5–8.0. The probe PHC can be applied in detecting Cu2+ in living cells monitored by confocal fluorescence microscopy imaging.",

keywords = "Bioimaging, Copper(II), Coumarin, Fluorescence turn-on",

author = "Wei, {Ling Fang} and Chen, {Chong Yan} and Lai, {Chia Kai} and Natesan Thirumalaivasan and Shu-Pao Wu",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

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doi = "10.1016/j.ymeth.2019.04.023",

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T1 - A nano-molar fluorescent turn-on probe for copper(II) detection in living cells

AU - Wei, Ling Fang

AU - Chen, Chong Yan

AU - Lai, Chia Kai

AU - Thirumalaivasan, Natesan

AU - Wu, Shu-Pao

PY - 2019/9/15

Y1 - 2019/9/15

N2 - The development of fluorescent probes to detect trace metal ions in biological samples has been in great need. Herein, a fluorescence turn-on sensor (PHC) was designed for highly selective detection of Cu2+ ions. The probe PHC shows weak fluorescence due to imine isomerization. With Cu2+, a significant blue emission due to Cu2+-induced oxidation of imine to a carboxylate group is observed. The turn-on process is observed with a 63-fold increase of fluorescence quantum yield (from 0.004 to 0.252). The emission intensity has a good linear relation at Cu2+ concentrations of 0–40 μM. The detection limit is estimated as 8 nM (S/N = 3). The maximum emission change induced by Cu2+ is found in the pH range of 6.5–8.0. The probe PHC can be applied in detecting Cu2+ in living cells monitored by confocal fluorescence microscopy imaging.

AB - The development of fluorescent probes to detect trace metal ions in biological samples has been in great need. Herein, a fluorescence turn-on sensor (PHC) was designed for highly selective detection of Cu2+ ions. The probe PHC shows weak fluorescence due to imine isomerization. With Cu2+, a significant blue emission due to Cu2+-induced oxidation of imine to a carboxylate group is observed. The turn-on process is observed with a 63-fold increase of fluorescence quantum yield (from 0.004 to 0.252). The emission intensity has a good linear relation at Cu2+ concentrations of 0–40 μM. The detection limit is estimated as 8 nM (S/N = 3). The maximum emission change induced by Cu2+ is found in the pH range of 6.5–8.0. The probe PHC can be applied in detecting Cu2+ in living cells monitored by confocal fluorescence microscopy imaging.

KW - Bioimaging

KW - Copper(II)

KW - Coumarin

KW - Fluorescence turn-on

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SN - 1046-2023

VL - 168

SP - 18

EP - 23

JO - Methods

JF - Methods

ER -

A nano-molar fluorescent turn-on probe for copper(II) detection in living cells

Abstract

Keywords

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