A mutant strain of microalga Chlorella sp. for the carbon dioxide capture from biogas

Chien Ya Kao, Sheng Yi Chiu, Tzu Ting Huang, Le Dai, Guan Hua Wang, Ching-Ping Tseng, Chiun-Hsun Chen, Chih-Sheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Scopus citations


To upgrade biogas produced from the anaerobic digestion of swine wastewater, an outdoor photobioreactor was established in this study. A mutant strain of microalga Chlorella sp. MM-2 was firstly isolated by ethyl methane sulfonate-induced random mutagenesis. The Chlorella sp. MM-2 grew in the presence of gas containing H 2S < 100 ppm, and the growth capacity of the microalgal culture aerated with 80% CH 4 was ∼70% that of the control culture (0% CH 4). In the field study, CO 2 capture efficiency of the Chlorella cultures, at a biomass concentration of 1.2 g L -1, from the desulfurized biogas (∼20% CO 2, ∼70% CH 4 and H 2S < 100 ppm) was approximate 70% on cloudy days and 80% on sunny days. CH 4 concentration in the effluent biogas from the Chlorella cultures was increased to approximate 84% on cloudy days and 87% on sunny days from its original 70%. The established outdoor photobioreactor system using a gas cycle-switching operation could be used as a CO 2 capture model for biogas upgrading.

Original languageEnglish
Pages (from-to)132-140
Number of pages9
JournalBiomass and Bioenergy
StatePublished - Jan 2012


  • Biogas
  • Carbon dioxide
  • Chlorella
  • Methane
  • Microalga
  • Photobioreactor


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