A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite

Kuan Yi Li; Ping Hsuan Lu; Bo Hsin Wu; Shu Pao Wu

doi:10.1016/j.dyepig.2022.110521

A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite

Kuan Yi Li, Ping Hsuan Lu, Bo Hsin Wu, Shu Pao Wu^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

As a reactive oxygen species (ROS), endogenous peroxynitrite is closely involved in several pathophysiological processes. The development of a peroxynitrite probe would be necessary to clarify the role of peroxynitrite in pathophysiology. In this study, a new fluorescent probe TPPT was synthesized to detect peroxynitrite with high specificity and sensitivity. The results demonstrated that TPPT exhibits a fluorescence turn-on response to peroxynitrite because of the peroxynitrite-triggered oxidative reaction. The reaction product was analyzed by several methods, and the mechanism of the peroxynitrite-triggered oxidative reaction was discussed. The efficacy of TPPT for intracellular peroxynitrite imaging was demonstrated systematically. The results showed that TPPT can successfully visualize intracellular peroxynitrite in RAW264.7 cells, zebrafish, and live mouse tissues.

Original language	English
Article number	110521
Journal	Dyes and Pigments
Volume	205
DOIs	https://doi.org/10.1016/j.dyepig.2022.110521
State	Published - Sep 2022

Keywords

Cell imaging
Fluorescent probes
Peroxynitrite
Zebrafish imaging

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10.1016/j.dyepig.2022.110521

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title = "A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite",

abstract = "As a reactive oxygen species (ROS), endogenous peroxynitrite is closely involved in several pathophysiological processes. The development of a peroxynitrite probe would be necessary to clarify the role of peroxynitrite in pathophysiology. In this study, a new fluorescent probe TPPT was synthesized to detect peroxynitrite with high specificity and sensitivity. The results demonstrated that TPPT exhibits a fluorescence turn-on response to peroxynitrite because of the peroxynitrite-triggered oxidative reaction. The reaction product was analyzed by several methods, and the mechanism of the peroxynitrite-triggered oxidative reaction was discussed. The efficacy of TPPT for intracellular peroxynitrite imaging was demonstrated systematically. The results showed that TPPT can successfully visualize intracellular peroxynitrite in RAW264.7 cells, zebrafish, and live mouse tissues.",

keywords = "Cell imaging, Fluorescent probes, Peroxynitrite, Zebrafish imaging",

author = "Li, {Kuan Yi} and Lu, {Ping Hsuan} and Wu, {Bo Hsin} and Wu, {Shu Pao}",

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year = "2022",

month = sep,

doi = "10.1016/j.dyepig.2022.110521",

language = "English",

volume = "205",

journal = "Dyes and Pigments",

issn = "0143-7208",

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TY - JOUR

T1 - A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite

AU - Li, Kuan Yi

AU - Lu, Ping Hsuan

AU - Wu, Bo Hsin

AU - Wu, Shu Pao

PY - 2022/9

Y1 - 2022/9

N2 - As a reactive oxygen species (ROS), endogenous peroxynitrite is closely involved in several pathophysiological processes. The development of a peroxynitrite probe would be necessary to clarify the role of peroxynitrite in pathophysiology. In this study, a new fluorescent probe TPPT was synthesized to detect peroxynitrite with high specificity and sensitivity. The results demonstrated that TPPT exhibits a fluorescence turn-on response to peroxynitrite because of the peroxynitrite-triggered oxidative reaction. The reaction product was analyzed by several methods, and the mechanism of the peroxynitrite-triggered oxidative reaction was discussed. The efficacy of TPPT for intracellular peroxynitrite imaging was demonstrated systematically. The results showed that TPPT can successfully visualize intracellular peroxynitrite in RAW264.7 cells, zebrafish, and live mouse tissues.

AB - As a reactive oxygen species (ROS), endogenous peroxynitrite is closely involved in several pathophysiological processes. The development of a peroxynitrite probe would be necessary to clarify the role of peroxynitrite in pathophysiology. In this study, a new fluorescent probe TPPT was synthesized to detect peroxynitrite with high specificity and sensitivity. The results demonstrated that TPPT exhibits a fluorescence turn-on response to peroxynitrite because of the peroxynitrite-triggered oxidative reaction. The reaction product was analyzed by several methods, and the mechanism of the peroxynitrite-triggered oxidative reaction was discussed. The efficacy of TPPT for intracellular peroxynitrite imaging was demonstrated systematically. The results showed that TPPT can successfully visualize intracellular peroxynitrite in RAW264.7 cells, zebrafish, and live mouse tissues.

KW - Cell imaging

KW - Fluorescent probes

KW - Peroxynitrite

KW - Zebrafish imaging

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U2 - 10.1016/j.dyepig.2022.110521

DO - 10.1016/j.dyepig.2022.110521

M3 - Article

AN - SCOPUS:85132915000

SN - 0143-7208

VL - 205

JO - Dyes and Pigments

JF - Dyes and Pigments

M1 - 110521

ER -

A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite

Abstract

Keywords

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