A mitochondria-targeting near-infrared fluorescent probe for the in vivo detection of peroxynitrite

Kuan Yi Li, Ping Hsuan Lu, Bo Hsin Wu, Shu Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

As a reactive oxygen species (ROS), endogenous peroxynitrite is closely involved in several pathophysiological processes. The development of a peroxynitrite probe would be necessary to clarify the role of peroxynitrite in pathophysiology. In this study, a new fluorescent probe TPPT was synthesized to detect peroxynitrite with high specificity and sensitivity. The results demonstrated that TPPT exhibits a fluorescence turn-on response to peroxynitrite because of the peroxynitrite-triggered oxidative reaction. The reaction product was analyzed by several methods, and the mechanism of the peroxynitrite-triggered oxidative reaction was discussed. The efficacy of TPPT for intracellular peroxynitrite imaging was demonstrated systematically. The results showed that TPPT can successfully visualize intracellular peroxynitrite in RAW264.7 cells, zebrafish, and live mouse tissues.

Original languageEnglish
Article number110521
JournalDyes and Pigments
Volume205
DOIs
StatePublished - Sep 2022

Keywords

  • Cell imaging
  • Fluorescent probes
  • Peroxynitrite
  • Zebrafish imaging

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