A miniaturized optically-induced fluorescence-activated cell sorter

S. M. Yang; M. Y. Ku; T. H. Punde; R. J. Chen; S. Y. Tseng; T. M. Yu; H. P. Huang; M. H. Liu; Long Hsu; C. H. Liu

doi:10.1109/TRANSDUCERS.2011.5969621

A miniaturized optically-induced fluorescence-activated cell sorter

S. M. Yang^*, M. Y. Ku, T. H. Punde, R. J. Chen, S. Y. Tseng, T. M. Yu, H. P. Huang, M. H. Liu, Long Hsu, C. H. Liu

^*Corresponding author for this work

Department of Electrophysics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120m distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.

Original language	English
Title of host publication	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages	326-329
Number of pages	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969621
State	Published - 1 Sep 2011
Event	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China Duration: 5 Jun 2011 → 9 Jun 2011

Publication series

Name	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/Territory	China
City	Beijing
Period	5/06/11 → 9/06/11

Keywords

cell sorting
dielectrophoresis
Light-induced optoelectroosmosis flow
photoconductivity
TiOPc

Access to Document

10.1109/TRANSDUCERS.2011.5969621

Cite this

Yang, S. M., Ku, M. Y., Punde, T. H., Chen, R. J., Tseng, S. Y., Yu, T. M., Huang, H. P., Liu, M. H., Hsu, L., & Liu, C. H. (2011). A miniaturized optically-induced fluorescence-activated cell sorter. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 326-329). Article 5969621 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969621

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title = "A miniaturized optically-induced fluorescence-activated cell sorter",

abstract = "We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120m distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.",

keywords = "cell sorting, dielectrophoresis, Light-induced optoelectroosmosis flow, photoconductivity, TiOPc",

author = "Yang, {S. M.} and Ku, {M. Y.} and Punde, {T. H.} and Chen, {R. J.} and Tseng, {S. Y.} and Yu, {T. M.} and Huang, {H. P.} and Liu, {M. H.} and Long Hsu and Liu, {C. H.}",

year = "2011",

month = sep,

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doi = "10.1109/TRANSDUCERS.2011.5969621",

language = "English",

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series = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11",

pages = "326--329",

booktitle = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11",

note = "2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 ; Conference date: 05-06-2011 Through 09-06-2011",

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Yang, SM, Ku, MY, Punde, TH, Chen, RJ, Tseng, SY, Yu, TM, Huang, HP, Liu, MH, Hsu, L & Liu, CH 2011, A miniaturized optically-induced fluorescence-activated cell sorter. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969621, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp. 326-329, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 5/06/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969621

A miniaturized optically-induced fluorescence-activated cell sorter. / Yang, S. M.; Ku, M. Y.; Punde, T. H. et al.
2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 326-329 5969621 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Yang, S. M.

AU - Ku, M. Y.

AU - Punde, T. H.

AU - Chen, R. J.

AU - Tseng, S. Y.

AU - Yu, T. M.

AU - Huang, H. P.

AU - Liu, M. H.

AU - Hsu, Long

AU - Liu, C. H.

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N2 - We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120m distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.

AB - We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120m distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.

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KW - dielectrophoresis

KW - Light-induced optoelectroosmosis flow

KW - photoconductivity

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T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

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BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

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Y2 - 5 June 2011 through 9 June 2011

ER -

Yang SM, Ku MY, Punde TH, Chen RJ, Tseng SY, Yu TM et al. A miniaturized optically-induced fluorescence-activated cell sorter. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 326-329. 5969621. (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). doi: 10.1109/TRANSDUCERS.2011.5969621

A miniaturized optically-induced fluorescence-activated cell sorter

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Keywords

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