A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation

Ho Ching Hsiao, Po Sheng Hu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Imaging adult zebrafish’s cardiac organ is often hampered by the limited volume of the internal cavity. This research study aims to design, manufacture and apply a miniature illumination probe amended with an aluminum-coated polydimethylsiloxane (PDMS) concave cylindrical mirrors as well as 3D-printed casings for probe assembly in a light-sheet fluorescence microscopy to explore its applicability of visualizing adult zebrafish’s ventricle. The system was calibrated and characterized. The experimental results successfully demonstrated the tracking of the ventricular regeneration in female adult zebrafish at multiple time points and can be conducive to other heart-related research in in vivo settings.

Original languageEnglish
Article number2293284
JournalInternational Journal of Optomechatronics
Volume18
Issue number1
DOIs
StatePublished - 2024

Keywords

  • 3D printing
  • adult zebrafish
  • in vivo monitoring
  • light-sheet fluorescence microscopy
  • Miniature illumination probe
  • ventricular resection

Fingerprint

Dive into the research topics of 'A miniature illumination probe in lightsheet fluorescence microscopy for real-time imaging injured adult zebrafish’s ventricular recuperation'. Together they form a unique fingerprint.

Cite this