A fully integrated system for cell/particle sorting in a microfluidic device utilizing an optical tweezing and DIP recognition approach

Yu Sheng Chien, Che Hsin Lin*, Fu Jen Kao, Cheng Wen Ko

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

This paper proposes a novel microfluidic system for cell/microparticle recognition and manipulation utilizing a digital image processing technique (DIP) controlled optical tweezer under microfluidic configuration. Cell/microparticle samples are firstly electrokinetically sorted in a microfluidic channel and pass through an image detection region. Digital image processing technique is used to count and recognize the cell/particle samples and then sends control signals to generate laser pulses to manipulate the target cell/particles optically. The optical tweezer system is capable of catching, moving and switching the target cells within the microfluidic channel. The trapping force of the optical tweezer is also demonstrated utilizing the relationship between Stocks-drag force of microparticles and the applied electroosmotic flow. The proposed system provides a simple but high-performance solution for microparticle manipulation in a microfluidic device.

Original languageEnglish
Title of host publicationProgress on Advanced Manufacture for Micro/Nano Technology 2005 - Proceedings of the 2005 International Conference on Advanced Manufacture
PublisherTrans Tech Publications Ltd.
Pages643-648
Number of pages6
EditionPART 1
ISBN (Print)0878499903, 9780878499908
DOIs
StatePublished - 2006
Event2005 International Conference on Advanced Manufacture, ICAM2005 - Taipei, R.O.C., Taiwan
Duration: 28 Nov 20052 Dec 2005

Publication series

NameMaterials Science Forum
NumberPART 1
Volume505-507
ISSN (Print)0255-5476
ISSN (Electronic)1662-9752

Conference

Conference2005 International Conference on Advanced Manufacture, ICAM2005
Country/TerritoryTaiwan
CityTaipei, R.O.C.
Period28/11/052/12/05

Keywords

  • Cell sorting
  • Digital image processing
  • Microfluidic
  • Optical tweezing

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