A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells

Chien Ju Chu; Guan Syuan Wu; Ho I. Ma; Parthiban Venkatesan; Natesan Thirumalaivasan; Shu Pao Wu

doi:10.1016/j.saa.2020.118234

A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells

Chien Ju Chu, Guan Syuan Wu, Ho I. Ma, Parthiban Venkatesan, Natesan Thirumalaivasan, Shu Pao Wu^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.

Original language	English
Article number	118234
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	233
DOIs	https://doi.org/10.1016/j.saa.2020.118234
State	Published - 5 Jun 2020

Keywords

BODIPY
Cell imaging
Fluorescent probe
HOCl

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10.1016/j.saa.2020.118234

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title = "A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells",

abstract = "Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.",

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author = "Chu, {Chien Ju} and Wu, {Guan Syuan} and Ma, {Ho I.} and Parthiban Venkatesan and Natesan Thirumalaivasan and Wu, {Shu Pao}",

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doi = "10.1016/j.saa.2020.118234",

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T1 - A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells

AU - Chu, Chien Ju

AU - Wu, Guan Syuan

AU - Ma, Ho I.

AU - Venkatesan, Parthiban

AU - Thirumalaivasan, Natesan

AU - Wu, Shu Pao

PY - 2020/6/5

Y1 - 2020/6/5

N2 - Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.

AB - Hypochlorous acid has played several functions in the biological system. However, excess HOCl can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCl is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells.

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KW - Cell imaging

KW - Fluorescent probe

KW - HOCl

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ER -

A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells

Abstract

Keywords

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