TY - GEN
T1 - 3D pH measurement for cell culture using CT scan
AU - Higashino, Nobuya
AU - Takayama, Toshio
AU - Ito, Hiroaki
AU - Horade, Mitsuhiro
AU - Tsai, Chia-Hung
AU - Kaneko, Makoto
PY - 2018/2/28
Y1 - 2018/2/28
N2 - In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.
AB - In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.
UR - http://www.scopus.com/inward/record.url?scp=85050466382&partnerID=8YFLogxK
U2 - 10.1109/MHS.2017.8305213
DO - 10.1109/MHS.2017.8305213
M3 - Conference contribution
AN - SCOPUS:85050466382
T3 - MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
SP - 1
EP - 3
BT - MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017
Y2 - 3 December 2017 through 6 December 2017
ER -