3-benzoyl-2-quinolinecarboxaldehyde: A novel fluorogenic reagent for the high-sensitivity chromatographic analysis of primary amines

Stephen C. Beale, You Zung Hsieh, Joseph C. Savage, Donald Wiesler, Milos Novotny*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

3-Benzoyl-2-quinolinecarboxaldehyde has been synthesized and characterized for use as a precolumn fluorogenic reagent for the ultrahigh sensitivity determination of primary amines by micro-column liquid chromatography with laser-induced fluorescence detection. The reaction conditions and the spectral properties of the derivatives were investigated with standard amino-acids. The detection limits, with an HeCd laser operated at 442 nm, are in the low femtogram range. The linear dynamic range is at least three orders of magnitude. The separation of a standard amino-acid mixture and the high-sensitivity analysis of a hydrolysed protein sample are demonstrated.

Original languageEnglish
Pages (from-to)321-325
Number of pages5
JournalTalanta
Volume36
Issue number1-2
DOIs
StatePublished - 1989

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